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Medicinal Chemistry
ISSN (Print): 1573-4064
ISSN (Online): 1875-6638
Epub Full Text Article
DOI: 10.2174/1573406410666140613152434      Price:  $95









Seeds of Peganum harmala L. Chemical Analysis, Antimalarial and Antioxidant Activities, and Cytotoxicity Against Human Breast Cancer Cells

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Author(s): Naziha Chabir, Hany Ibrahim, Mehrez Romdhane, Alexis Valentin, Béatrice Moukarzel, Mohamed Mars and Jalloul Bouajila
Abstract:
The present study evaluated the levels of total phenolics, flavonoids, tannins and anthocyanins from Peganum harmala L. seeds and determined their antioxidant, antiplasmodial and anticancer potentials. Antioxidant activity was determined by DPPH and ABTS assays. Extracts of P. harmala seeds from Oudref and Djerba (two places in Tunisia) were obtained by successive extraction solvents: petroleum ether, chloroform, ethyl acetate, ethanol and water. Their composition was evaluated for phenolics (gallic acid equivalent 2.48 to 72.52 g/kg), tannins (catechin equivalent 0 to 25.27 g/kg), anthocyanins (cyanidin equivalent 0 to 20.56 mg/kg) and flavonoids (quercetin equivalent 0 to 3.12 g/kg). Ethanolic extract exerted the highest activities against a chloroquine-resistant strain of Plasmodium falciparum (IC50=23 mg/L), against human breast cancer cells MCF7 (IC50=32 mg/L) and against free radical (IC50=19.09±3.07 mg/L). Correlations were studied between each chemical family and the three activities. Total phenolics content exhibited the highest correlation with antiplasmodial activity (R2=0.92) and with anticancer activity (R2=0.86), respectively
Keywords:
Antimalarial, antioxidant, cytotoxic, extracts, Peganum harmala L., phenolics
Affiliation:
Université de Toulouse, Université Paul Sabatier, Faculté de pharmacie de Toulouse, Laboratoire des IMRCP UMR CNRS 5623, 118 route de Narbonne, F-31062 Toulouse, France