Determination of the New Antiplatelet Agent Ticagrelor in Tablets by Stability-Indicating HPLC Method
Caren Gobetti, Rubia Lazzaretti Pereira, Andreas Sebastian Loureiro Mendez and Cássia Virginia Garcia
Affiliation: Av. Ipiranga 2752, Sala 406. CEP 90610-000, Porto Alegre-RS, Brazil.
Ticagrelor is an orally active, reversibly bind of the P2Y12 receptor that can prevent ADP-mediated platelet activation
and has a faster onset of action than clopidogrel. A stability-indicating HPLC method for the determination of ticagrelor
in coated tablets was developed and validated. Chromatographic analysis was performed in a Shimadzu liquid
chromatograph, equipped with Phemomenex® C18 column (250 x 4.6 mm, 5 μm), using a mobile phase composed of acetonitrile:
water with 0.5% triethylamine (57:43 v/v) pH 7.0, at 0.7 ml/min flow rate and injection volume of 20 μl. Under
the conditions established, the method demonstrated to be specific, without interference from formulation excipients, stability-
indicating, linear (r = 0.9990) in the concentration range of 45.0 to 105.0 μg/ml, precise (RSD=0.71), accurate
(99.61% of mean recovery) and robust. It can be successfully applied to ticagrelor tablets quality control, being adequate
for routine analysis.
Keywords: Antiplatelet, HPLC, quality control, stability-indicating assay, tablets, ticagrelor.
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