Although BSA Au clusters fluoresce in red region (λmax: 650nm), they are of limited use due to low fluorescence
quantum yield (~6%). Here we report an enhanced fluorescence imaging application of fluorescent bio-nano probe
BSA Au clusters using multipulse excitation scheme. Multipulse excitation takes advantage of long fluorescence lifetime
(> 1µs) of BSA Au clusters and enhances its fluorescence intensity 15 times over short lived cellular auto-fluorescence.
Moreover we have also shown that by using time gated detection strategy signal (fluorescence of BSA Au clusters) to
noise (auto-fluorescence) ratio can be increased by 30 fold. Thereby with multipulse excitation long lifetime probes can be
used to develop biochemical assays and perform optical imaging with zero background.
Keywords: Autofluorescence, BSA Au clusters, long lifetime, multipulse excitation, signal to noise.
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