BSA Au Clusters as a Probe for Enhanced Fluorescence Detection Using Multipulse Excitation Scheme

Author(s): Sangram L. Raut , Ryan Rich , Rafal Fudala , R. Kokate , J.D. Kimball , Julian Borejdo , Jamboor K. Vishwanatha , Zygmunt Gryczynski , Ignacy Gryczynski .

Journal Name: Current Pharmaceutical Biotechnology

Volume 14 , Issue 13 , 2013

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Abstract:

Although BSA Au clusters fluoresce in red region (λmax: 650nm), they are of limited use due to low fluorescence quantum yield (~6%). Here we report an enhanced fluorescence imaging application of fluorescent bio-nano probe BSA Au clusters using multipulse excitation scheme. Multipulse excitation takes advantage of long fluorescence lifetime (> 1µs) of BSA Au clusters and enhances its fluorescence intensity 15 times over short lived cellular auto-fluorescence. Moreover we have also shown that by using time gated detection strategy signal (fluorescence of BSA Au clusters) to noise (auto-fluorescence) ratio can be increased by 30 fold. Thereby with multipulse excitation long lifetime probes can be used to develop biochemical assays and perform optical imaging with zero background.

Keywords: Autofluorescence, BSA Au clusters, long lifetime, multipulse excitation, signal to noise.

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Article Details

VOLUME: 14
ISSUE: 13
Year: 2013
Page: [1139 - 1144]
Pages: 6
DOI: 10.2174/1389201015666140523161038
Price: $58

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