Protein Content Analysis and Antimicrobial Activity of the Crude Venom of Montivipera bornmuelleri; a Viper from Lebanon
Fawaz El Omar,
Viperidae snakes venoms represent a source of efficient bioactive components that have already led to the development
of several new drugs. In this work, we analyzed the protein content of the Montivipera bornmuelleri crude
venom using LC-ESI-MS, sephadex G-75 gel filtration and SDS-PAGE and demonstrated the presence of proteins with
molecular masses corresponding to metalloprotease III, serine-protease and PLA2 in three fractions collected after gel filtration.
Equally, we examined the antimicrobial effect of the venom that showed an important potency, as bactericidal
agent, based on MBC and MIC values obtained, against Staphylococcus aureus and Morganella morganii bacteria. However,
no activity was registered against Enterococcus faecalis, being the most resistant bacteria, neither against Aspergillus
flavus and Penicillium digitatum fungal. Furthermore, on eleven other bacterial strains and the Candida albicans fungus,
the venom has shown an intermediate efficacy by slightly reducing the growth. Our data concerning the Montivipera
bornmuelleri venom give evidence of a rich and complex content aiding the exploration of new bioactive molecules for
Keywords: Antibacterial and antifungal activities, crude venom, Montivipera bornmuelleri, proteome analysis.
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