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Current Pharmaceutical Biotechnology
ISSN (Print): 1389-2010
ISSN (Online): 1873-4316
Epub Full Text Article
DOI: 10.2174/1389201015666140519110616      Price:  $95









miR-21, miR-106b and miR-375 as Novel Potential Biomarkers for Laryngeal Squamous Cell Carcinoma

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Author(s): Y u Xing, W u Yibo, L iu Yang, Hongxia Deng, Zhisen Shen, Bingxiu Xiao and Junming Guo
Abstract:
MicroRNAs (miRNAs) are endogenously expressed noncoding RNAs, which are involved in carcinogenesis. Laryngeal squamous cell carcinoma (LSCC) represents the largest subgroup of squamous cell carcinoma in head and neck. Whether miRNAs can be used in the diagnosis of LSCC is largely unknown. The aim of this study was to identify whether miRNAs could be serve as novel potential biomarkers for LSCC diagnosis. The expression levels of three miRNAs, miR-21, miR-106b and miR-375, in paired-LSCC tissues and adjacent non-tumor tissues were first quantitatively analyzed by reverse transcription-polymerase chain reaction. Then, the relationships between their levels and clinicopathological factors of patients with LSCC were further explored. We found that these miRNAs’ expression levels were significantly different between LSCC tissues and adjacent normal tissues. miR-21 and miR-106b were up-regulated in cancer tissues (P=0.0012 and P<0.001, respectively), while miR-375 down-regulated (P<0.001). Moreover, we found that miR-21 and miR-106b levels were significantly increased in poorly (G3)/moderately differentiated (G2) cancer tissues comparing with well differentiated (G1) and dysplasia (D) tissues (P<0.001, respectively). Their levels were also positively associated with TNM stages (P<0.01) and lymph node metastasis (P<0.05). However, miR-375 expression level was only found negatively associated with TNM stages. Our preliminary data suggest that miR-21, miR-106b and miR-375 may be novel potential biomarkers for LSCC diagnosis
Keywords:
Laryngeal squamous cell carcinoma, MicroRNAs, MiR-21, MiR-106b, MiR-375, tumor markers
Affiliation:
Ningbo University School of Medicine, 818 Fenghua Road, Ningbo 315211, China