Enhancement of Fermentative Hydrogen Production from Beverage Wastewater via Bioaugmentation and Statistical Optimization
Periyasamy Sivagurunathan, Kumar Gopalakrishnan and Chiu-Yue Lin
Affiliation: Green Energy Development Center, Feng Chia University, Taichung 40724, Taiwan.
In this study an attempt was made to enhance anaerobic hydrogen production from beverage wastewater
(BWW) by augmenting anaerobic enriched mixed cultures (EMC) with facultative Enterobacteriaceae strains (E. Coli
XL1-BLUE, Enterobacter cloacae) under mesophilic temperature 37°C, pH 5.5 and substrate concentration of 10 gglucose
equivalent /L. Among the bioaugmented cultures, E. cloacae+EMC led to the peak hydrogen production rate (HPR) of 2250
mL/L-d. Furthermore, the variables substrate concentration and pH was statistically optimized using response surface
methodology (RSM). Results show that the predicted maximum HPR of 3096 mL/L-d was achieved under optimal operation
conditions: substrate concentration 25.6 g glucose equivalent /L and pH 5.7. Verification experimental results showed HPR
of 3044 mL/L-d, reveals that predicted results were closure with the experimental data and proved experimental design
optimization processes are more feasible techniques for biohydrogen production. PCR-DGGE analysis indicates that Clostridium
sp. and Enterobacter sp. were appeared under optimal conditions.
Keywords: Beverage wastewater, bioaugmentation, biohydrogen, enriched mixed culture, hydrogen production rate, PCRDGGE.
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