Purification of Paraoxonase Enzyme From the Sera of Patients with Behcet’s Disease and Analyzing the Effects of the Drugs Containing Imuran (Azathioprine), Prednisolone (Methylprednisolone) and Colchium (Colchicine)
Nazan Demir, Hayrunnisa Nadaroglu, Asuman Ozkan, Esen Tasgin, Ceyhun Isik and Yasar Demir
Affiliation: Mugla University, Faculty of Science, Department of Chemistry, 48000, Mugla, Turkey.
In this study, serum samples from 50 patients with the diagnosis of Behcet’s disease and 20
healthy volunteers were analyzed. The study consists of three parts. In the first part, paraoxonase (PON)
activities were determined in the serum samples of 50 patients with Behcet’s disease and 20 healthy people.
In the second part, equal volumes of serum samples from 50 patients were pooled and PON enzymes were
purified by using Sepharose-4B-L-tyrosine tyrosine-1-naphtylamine affinity column. Optimum temperature,
optimum pH, Vmax and Km values of the pure enzymes were determined. The same purification procedure
was also performed in the serum samples of 20 healthy people.
Electrophoretic mobility was observed (via SDS-PAGE) in the PON enzymes that were purified from the serum samples
of patients with Behcet’s disease and healthy people. In the third part, in vitro effects of drugs containing azathioprine,
methylprednisolone and colchicine that have already been used for the treatment of Behcet’s disease were tested on the
PON enzymes of the patients with Behcet’s disease and control group.
IC50 values and Ki constant values were measured and inhibition types were determined for the drugs containing
azathioprine, methylprednisolone and colchicine that have already been used for the treatment of the Behcet’s disease and
demonstrate in vitro inhibition effects.
Keywords: Azathioprine, behcet disease, colchicine, methylprednisolone, paraoxonase, purification.
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