Current Chemical Biology

Fernando Albericio  
School of Chemistry
Yachay Tech University
Yachay City of Knowledge, Urcuqui
Ecuador

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Simulating the Bacterial Chemotaxis Pathway at High Spatio-temporal Detail

Author(s): Sven Sewitz and Karen Lipkow

Affiliation: Babraham Institute, Nuclear Dynamics (B570), Babraham Research Campus, Cambridge CB22 3AT, UK.

Keywords: Bacterial chemotaxis, cellular signalling, particle based simulation, protein gradients, review, smoldyn.

Abstract:

We have used the Smoldyn software to create spatially detailed models of the Escherichia coli chemotaxis pathway. With it we can follow signalling reactions at high spatio-temporal resolution, observe the formation of gradients of phospho-proteins as well as total protein and analyse the effects of macromolecular crowding on signalling. It has enabled us to propose new regulatory elements of the signalling pathway, which are mediated through the dynamic localisation and activity of the CheZ phosphatase. We used the Smoldyn software to model quantitative fluorescent microscopy data and to determine diffusion coefficients and binding affinities. We can reliably gain information obtained under conditions with high levels of experimental noise. Smoldyn can accommodate various cellular architectures, and we show that cell shape becomes an integral part of the signalling process.

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Article Details

VOLUME: 7
ISSUE: 3
Page: [214 - 223]
Pages: 10
DOI: 10.2174/221279680703140508101810