Current Alzheimer Research

Prof. Debomoy K. Lahiri  
Department of Psychiatry, Indiana University School of Medicine
Neuroscience Research Center
Indianapolis, IN 46202


RCAN1 Increases Aβ Generation by Promoting N-glycosylation via Oligosaccharyltransferase

Author(s): Tan Wang, Heng Liu, Yun Wang, Changqing Liu and Xiulian Sun

Affiliation: Qilu Hospital of Shandong University, 107 Wenhua Xi Road, Jinan, Shandong, 250012, P. R. China

Keywords: Aβ, alzheimer’s disease, β-secretase, γ-secretase, N-glycosylation, oligosaccharyltransferase, RCAN1


Glycosylation is one of the major post-translational modifications, required for proper folding and functions of glycoproteins. N-glycosylation in ER is mediated by oligosaccharyltransferase (OST), an enzyme complex transferring preassembled oligosaccharide to asparagine residues of nascent polypeptide chain. Our study here indicates that regulator of calcineurin 1 (RCAN1) can enhance N-glycosylation in ER, therefore elevates the activities of β- and γ-secretase and markedly increases Aβ production. We found that RCAN1 stabilizes OST by interacting with OST component ribophorinI (RPN I). RCAN1 enhanced glycosylation of membrane proteins and glycosylation sequon GNSTVT, but has no effect on transferrin whose glycosylation was only affected by OST catalytic subunit STT3A, suggesting the effect of RCAN1 is associated with RPN I in facilitating substrate delivery. Our previous studies have shown that RCAN1 was increased in AD brains and RCAN1 overexpression induced neuronal apoptosis. Here our study showed that RCAN1 further contributes to AD pathogenesis by increasing N-glycosylation and Aβ production.

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Article Details

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Pages: 8
DOI: 10.2174/1567205011888140411163936