Characterization of Stem-Like Cells Directly Isolated from Freshly Resected Laryngeal Squamous Cell Carcinoma Specimens
Ilknur Suer, Omer Faruk Karatas, Betul Yuceturk, Mehmet Yilmaz, Gulgun Guven, Buge Oz, Harun Cansiz and Mustafa Ozen
Affiliation: Istanbul University, Cerrahpasa Medical School, Department of Medical Genetics, Fatih, Istanbul, Turkey.
Larynx cancer (LCa) is an aggressive malignancy, which is the second most common malignant neoplasm of
head and neck squamous cell carcinoma. Its incidences have been reported to increase and therapeutic options mostly fail
to give positive clinical response especially for the advanced LCa cases. In this study we aimed to isolate stem-like cells
from freshly resected LCa tumor specimens and characterize them by quantitative real time PCR (qRT-PCR) for expression
of cancer stem cell markers including SOX2, OCT4, KLF4, ABCG2, CXCR4 and CD44. Our results showed that
CD133(high) cells directly isolated from freshly resected tumor specimens exhibit elevated levels of SOX2, OCT4 and
KLF4, and have increased expression levels of ABCG2 and CXCR4, which were associated with resistance of tumors to
regular chemotherapeutic reagents. In conclusion, this study offers a useful approach utilizing CD133 to isolate stem cells
directly from fresh tissues, which gives the opportunity to develop novel therapeutic tools specifically targeting these cells
through their further characterization.
Keywords: CD133, chemoresistance, laryngeal squamous cell carcinoma, MACS, qRT-PCR, stem-like cells.
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