Strategic siRNA Screening Approaches to Target Cancer at the Cancer Research UK Beatson Institute
Emma J. Shanks.
The RNAi Screening Facility at the Cancer Research UK Beatson Institute combines siRNA
genome-wide screening with drug screening coupled with High Content Imaging and fluorescence-based
phenotypic assays to target multiple types of cancer. Here, we describe the infrastructure of the Facility
and the approaches we utilise. We also share our experiences in running such a facility and developing
and executing screening campaigns, with particular regard to high content multiparametric analysis, data
management and statistical analysis.
Keywords: Cancer, high content analysis, phenotypic screening, RNAi screening, statistical analysis.
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