The Sheffield RNAi Screening Facility (SRSF) (www.rnai.group.shef.ac.uk) was
established in 2008 with Wellcome Trust and University of Sheffield funding, with the task to
provide the first UK RNAi screening resource for academic groups interested in identifying genes
required in a diverse range of biological processes using Drosophila cell culture. The SRSF has
carried out a wide range of screens varying in sizes from bespoke small-scale libraries, targeting a
few hundred genes, to high-throughput, genome-wide studies. The SRSF has grown and improved
with a dedicated partnership of its academic customers based mainly in the UK. We are part of the
UK Academics Functional Genomics Network, participating in organizing an annual meeting in
London and are part of the University of Sheffield’s D3N (www.d3n.org.uk), connecting
academics, biotech and pharmaceutical companies with a multidisciplinary network in Drug Discovery and Development.
Recently, the SRSF has been funded by the Yorkshire Cancer Research Fund to perform genome-wide RNAi screens
using human cells as part of a core facility for regional Yorkshire Universities and screens are now underway. Overall the
SRSF has carried out more than 40 screens from Drosophila and human cell culture experiments.