Abstract
During the past several years, studies on the protein aggregation process in the presence of cosolvents/ co-solutes have been looked into which provides significant insight in the stability of proteins in a crowded cellular milieu. Here, in the present report we have investigated the fibrillation of human serum albumin (HSA) under the mixed aqueous-ethanol solvent conditions at two different temperatures (37 °C and 65 °C). Self-association of protein was monitored using various spectroscopic and microscopic techniques. Results obtained from detailed investigation have shown that fibrillation of human serum albumin is favored at higher temperature (65 °C) at lower ethanol concentration. However, at 37 °C comparatively higher ethanol concentration is the prerequisite condition for fibrillation process to take place.
Keywords: Ethanol, fibrillation, human serum albumin, solvent, spectroscopy, temperature.
Protein & Peptide Letters
Title:Effect of Temperature and Solvent on Fibrillation of Human Serum Albumin
Volume: 22 Issue: 2
Author(s): Nitin K. Pandey, Sudeshna Ghosh, Debi R. Tripathy and Swagata Dasgupta
Affiliation:
Keywords: Ethanol, fibrillation, human serum albumin, solvent, spectroscopy, temperature.
Abstract: During the past several years, studies on the protein aggregation process in the presence of cosolvents/ co-solutes have been looked into which provides significant insight in the stability of proteins in a crowded cellular milieu. Here, in the present report we have investigated the fibrillation of human serum albumin (HSA) under the mixed aqueous-ethanol solvent conditions at two different temperatures (37 °C and 65 °C). Self-association of protein was monitored using various spectroscopic and microscopic techniques. Results obtained from detailed investigation have shown that fibrillation of human serum albumin is favored at higher temperature (65 °C) at lower ethanol concentration. However, at 37 °C comparatively higher ethanol concentration is the prerequisite condition for fibrillation process to take place.
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Pandey K. Nitin, Ghosh Sudeshna, Tripathy R. Debi and Dasgupta Swagata, Effect of Temperature and Solvent on Fibrillation of Human Serum Albumin, Protein & Peptide Letters 2015; 22 (2) . https://dx.doi.org/10.2174/0929866521666140320104409
DOI https://dx.doi.org/10.2174/0929866521666140320104409 |
Print ISSN 0929-8665 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5305 |
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