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Protein & Peptide Letters
ISSN (Print): 0929-8665
ISSN (Online): 1875-5305
Epub Full Text Article
DOI: 10.2174/0929866521666140320104409      Price:  $95









Effect of Temperature and Solvent on Fibrillation of Human Serum Albumin

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Author(s): Nitin K. Pandey, Sudeshna Ghosh, Debi Ranjan Tripathy and Swagata Dasgupta
Abstract:
The in vivo deposition of toxic proteinaceous aggregates with fibrillar morphology has been known to direct the pathogenesis of severe neurological disorders. The stability of proteins under the crowded cellular milieu might be crucial for the fate of proteins in terms of aggregation. Here in the present report, we have examined the effect of ethanol on fibrillation process of human serum albumin (HSA) at two different temperatures 37 °C and 65 °C. Different spectroscopic and microscopic techniques have been employed to study the propagation of fibrillation. Studies suggest that ethanol favors fibrillation of HSA and the effect was found to be more pronounced at elevated temperature
Keywords:
Ethanol, fibrillation, human serum albumin, solvent, spectroscopy, temperature
Affiliation:
Department of Chemistry, Indian Institute of Technology, Kharagpur, 721302, India