Isolation and Identification of a Phenol-Degrading Yeast JP-1 with High Activity and Its Degrading Characteristics and Kinetic
Fei Pan, Ai-Hua Xu, Qing-Fu Zeng, Xin Zhao and Hai-Liang Zhu
Affiliation: School of Life Science, Nanjing University, Nanjing 210093, China.
Keywords: Biodegradation, Candida tropicalis, kinetic, Haldane equation, phenol, wastewater treatment.
A yeast strain with high activity of phenol degradation was isolated from activated sludge of a wastewater
treatment plant in a coking chemical factory, and identified as Candida tropicalis according to the analysis of VITEK-32
and its 18SrDNA gene sequence. The strain designated as JP-1 was able to grow on phenol as a sole carbon and energy
source and it can degrade more than 90% of 1800 mg⋅L-1 phenol within 72h. The optimum pH was 6.0. Kinetic equation
of phenol degradation was obtained by regression using MATLAB software. The parameters of Haldane equation were
following: µmax=0.387 l⋅hr-1, Ks=4.20 mg⋅L-1, KiKi =465.6 mg⋅L-1. Cell grew was obviously inhibition by high
concentration phenol and the inhibition strengthen increased with phenol increasing.
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