Inclusion of Dietary Zeolite Reduces Aflatoxin B1 Levels in Household Bread Waste Used as Cattle Feed
M. Yavarmanesh, M. Sohrabi Balssini, M.R. Edalatian Dovom, F. Ghiamati Yazdi and J. Barouei
Affiliation: Department of Food Science & Technology, Faculty of Agriculture, Ferdowsi University of Mashhad (FUM).
Keywords: Zeolite, aflatoxin, reduction, household bread waste, cattle feed.
This study aimed to examine the efficacy of zeolite addition in the reduction of aflatoxin B1 (AFB1) levels in
household bread waste used as animal feed. Three levels of zeolite (1, 3 and 5%) were added to two dry bread waste samples
naturally contaminated by AFB1 at 11.48 and 4.8 ppm levels. Samples were then analyzed before zeolite treatment
(day 0) and after 7 and 14 days of storage for chemical (pH, moisture content and ash) and microbial (mesophilic bacteria
and molds) changes as well as aflatoxin levels relative to the control. Data analysis showed that aflatoxin levels significantly
decreased as zeolite and storage time increased, so that the lowest level of aflatoxin was found in sample treated
with 5% zeolite after 14 days of storage compared with the control (p<0.05). Such a trend was also observed in bacterial
and mold counts. However, storage time had less effect than zeolite. Chemical analyses of bread samples exhibited significant
increases in pH values and corresponding decreases in moisture and ash contents in all storage time points compared
with the control. These findings suggest zeolite addition as a viable method of reduction of AFB1 levels in household
bread waste used as animal feed.
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