Regulation and Quantification of Cellular Mitochondrial Morphology and Content

Author(s): Karl J. Tronstad, Marco Nooteboom, Linn I. H. Nilsson, Julie Nikolaisen, Maciek Sokolewicz, Sander Grefte, Ina K.N. Pettersen, Sissel Dyrstad, Fredrik Hoel, Peter H.G.M. Willems, Werner J.H. Koopman.

Journal Name: Current Pharmaceutical Design

Volume 20 , Issue 35 , 2014

Submit Manuscript
Submit Proposal

Abstract:

Mitochondria play a key role in signal transduction, redox homeostasis and cell survival, which extends far beyond their classical functioning in ATP production and energy metabolism. In living cells, mitochondrial content (“mitochondrial mass”) depends on the cell-controlled balance between mitochondrial biogenesis and degradation. These processes are intricately linked to changes in net mitochondrial morphology and spatiotemporal positioning (“mitochondrial dynamics”), which are governed by mitochondrial fusion, fission and motility. It is becoming increasingly clear that mitochondrial mass and dynamics, as well as its ultrastructure and volume, are mechanistically linked to mitochondrial function and the cell. This means that proper quantification of mitochondrial morphology and content is of prime importance in understanding mitochondrial and cellular physiology in health and disease. This review first presents how cellular mitochondrial content is regulated at the level of mitochondrial biogenesis, degradation and dynamics. Next we discuss how mitochondrial dynamics and content can be analyzed with a special emphasis on quantitative live-cell microscopy strategies.

Keywords: Mitochondrial biogenesis, mitochondrial dynamics, mitochondrial degradation, living cells, TMRM, microscopy, segmentation, image analysis.

Rights & PermissionsPrintExport Cite as


Article Details

VOLUME: 20
ISSUE: 35
Year: 2014
Page: [5634 - 5652]
Pages: 19
DOI: 10.2174/1381612820666140305230546
Price: $58

Article Metrics

PDF: 71