miR-143: A Novel Regulator of MyoD Expression in Fast and Slow Muscles of Siniperca chuatsi
L. Chen, P. Wu, X.-H. Guo, Y. Hu, Y.-L. Li, J. Shi, K.-Z. Wang, W.-Y. Chu and J.-S. Zhang
Affiliation: Department of Bioengineering and Environmental Science, Changsha University, Changsha, Hunan 410003, China.
The fast and slow skeletal muscle fibers in fish are separated to a much greater degree than in
mammals. MyoD is required for the maintenance of normal fiber type balance in muscles. So far, the upstream
regulatory factors of MyoD in terms of controlling muscle phenotype are poorly understood. In the present
study, we used Siniperca chuatsi as a model system and demonstrated that miR-143 expression was negatively
correlated with MyoD expression in the fast and slow muscles of S. chuatsi. The luciferase reporter assay
further verified the direct interaction between the miR-143 and MyoD. The miR-143 suppression also led to the
significant increase in MyoD and fast myosin heavy chain gene expression in vivo. Taken together, our studies
indicated that miRNA participates in controlling the performance of different muscle fiber types in vertebrates.
Keywords: microRNA, muscle fiber, MyoD, regulation, Siniperca chuatsi.
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