Regeneration in Tetrapleura tetraptera via Indirect Somatic Embryogenesis from Isolated Axillary Meristems
Jelili. T. Opabode and Oluyemisi A. Akinyemiju
Affiliation: Department of Crop Production and Protection, Obafemi Awolowo University, Ile-Ife, Nigeria.
Keywords: Fabaceae, plant regeneration, somatic embryogenesis, Tetrapleura tetraptera, woody legume.
To facilitate the use of biotechnological techniques for conservation, germplasm exchange and improvement of
Tetrapleura tetraptera, the study investigated in vitro plant regeneration via somatic embryogenesis from isolated axillary
meristem of the plant. Indirect primary somatic embryos were induced on MS basal medium supplemented with nine
different concentrations of 2,4-D. Pre-embryogenic calli were made to mature on MS basal medium containing BAP alone
or with four concentrations of NAA. Secondary somatic embryos were induced on MS basal medium supplemented with
nine different concentrations of 2,4-D. Maturation of the secondary pre-embryogenic calli took place on MS medium
fortified with eight concentrations of BAP. Medium fortified with 6.0 mg/l 2, 4-D had the highest frequency of primary
embryogenic callus (16.4%) and average number of primary immature embryo per callus (2.3). Culture media
supplemented with 0.6 mg/l BAP and 0.1 mg/l NAA recorded the highest primary embryogenesis (24.5%) and average
number of matured primary embryo per explant (2.8 embryos/explants). Medium fortified with 1.2 mg/l BAP gave the
highest secondary embryogenesis (72.4%) and average number of matured embryo per callus (3.1 embryos per callus).
Explants in medium receiving 1.0 mg/l BAP and 0.7 mg/l IBA recorded largest number of shoot-bud (27.6%) and average
number of shoot-bud per explant (2.4/explant) with vigorous and green appearance. Shoot elongation was greatest in
medium fortified with 1.0 mg/l BAP and 15.0 mg/l IBA with vigorous appearance. The largest number of roots
(4.3/plantlet) were formed by plantlets grown in medium supplemented with 1.0 mg/l BAP and 15.0 mg/l IBA with
normal appearance. The study concluded that indirect somatic embryos obtained from isolated axillary meristem with 2,4-
D, BAP, NAA were capable of regeneration into normal vigorous plants.
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