Gemcitabine Resistance is Associated with Epithelial-Mesenchymal Transition and Induction of HIF-1α in Pancreatic Cancer Cells
Rui Wang, Long Cheng, Jun Xia, Zishu Wang, Qiong Wu and Zhiwei Wang
Affiliation: Department of Medical Oncology, First Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui, 233004, China.
Keywords: Chemoresistance, EMT, gemcitabine, HIF-1α, pancreatic cancer.
Pancreatic cancer is one of the highly aggressive malignant diseases worldwide. To achieve better treatment
outcome of pancreatic cancer, in the current study we explore the underlying molecular mechanism of drug resistance in
pancreatic cancer cells. We found that resistance to gemcitabine is associated with epithelial-mesenchymal transition
(EMT) phenotype in a panel of pancreatic cancer cell lines. Notably, gemcitabine-resistant pancreatic cancer cells acquire
EMT phenotype. Moreover, gemcitabine-resistant cells have increased migration and invasion activities. Furthermore, we
observed the high expression of HIF-1α in gemcitabine-resistant cells. More importantly, inhibition of HIF-1α in
gemcitabine-resistant cells caused partial reversal of EMT phenotype, suggesting that HIF-1α was critically involved in
gemcitabine-resistant-mediated EMT. Therefore, targeting HIF-1α could be an effective strategy for the treatment of
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