Baicalin Induces Apoptosis of Gallbladder Carcinoma Cells in vitro via a Mitochondrial-Mediated Pathway and Suppresses Tumor Growth in vivo
Yi-Jun Shu, Run-Fa Bao, Xiang-Song Wu, Hao Weng, Qian Ding, Yang Cao, Mao-Lan Li, Jia-Sheng Mu, Wen-Guang Wu, Qi-Chen Ding, Tian-Yu Liu, Lin Jiang, Yun-Ping Hu, Zhu-Jun Tan, Peng Wang and Ying-Bin Liu
Affiliation: Laboratory of General Surgery and Department of General Surgery, Xinhua Hospital, Affiliated to Shanghai Jiao Tong University, School of Medicine, No. 1665 Kongjiang Road, Shanghai 200092, China.
Baicalin, the main active ingredient in the Scutellaria baicalensis (SB), is prescribed for the treatment of various inflammatory
diseases and tumors in clinics in China. In the present study, we evaluated the antitumor activity of baicalin for gallbladder carcinoma
and the underlying mechanisms both in vitro and in vivo. Our results indicate that baicalin induced potent growth inhibition, cell cycle
arrest, apoptosis and colony-formation inhibition in a dose-dependent manner in vitro. We observed inhibition of NF-κB nuclear
translocation, up-regulation of Bax and down-regulation of Bcl-2, as well as increased caspase-3 and caspase-9 expression after baicalin
treatment in vitro and in vivo, which indicates that the mitochondrial pathway was involved in baicalin-induced apoptosis. In addition,
daily intraperitoneally injection of baicalin (15, 30 and 60 mg/kg) for 21 days significantly inhibited the growth of NOZ cells xenografts
in nude mice, which improved the survival of baicalin-treated mice. In summary, baicalin exhibited a significant anti-tumor effect by
suppressing cell proliferation, promoting apoptosis, and inducing cell cycle arrest in vitro, and by suppressing tumor growth and
improving survival in vivo, which suggested that baicalin represents a novel therapeutic option for gallbladder carcinoma.
Keywords: Apoptosis, Baicalin, gallbladder carcinoma, mitochondrial-mediated pathway, survival rate, xenograft.
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