Abstract
Synthetic DNA molecules of 10-23 deoxyribozymes (Dz) are known to be efficient and site-specific agents for the RNA cleavage in a catalytic manner. To provide the penetration of Dz into cells, we have proposed the new delivery system for deoxyribozymes in the form of TiO2•PL-Dz nanocomposite. These nanocomposites consist of catalytically active Dz molecules noncovalently immobilized through the polylysine linker to TiO2 nanoparticles, which provide the penetration of Dz into cells. Deoxyribozymes in the prepared TiO2•PL-Dz nanocomposites were shown to retain their ability to cleave RNA targets albeit at a slower rate but with the same site-specificity and similar efficiency as free Dz. Unlike free Dz, the proposed TiO2•PL-Dz nanocomposites penetrate into cells without auxiliary actions.
Keywords: Delivery, deoxyribozymes, immobilization, nanocomposites, TiO2 nanoparticles.
Drug Delivery Letters
Title:TiO2~Deoxyribozyme Nanocomposites as Delivery System and Efficient Site-Specific Agents for Cleavage of RNA Targets
Volume: 4 Issue: 2
Author(s): Asya S. Levina, Marina N. Repkova, Nadezhda V. Shikina, Zinfer R. Ismagilov, Natalia V. Shatskaya and Valentina F. Zarytova
Affiliation:
Keywords: Delivery, deoxyribozymes, immobilization, nanocomposites, TiO2 nanoparticles.
Abstract: Synthetic DNA molecules of 10-23 deoxyribozymes (Dz) are known to be efficient and site-specific agents for the RNA cleavage in a catalytic manner. To provide the penetration of Dz into cells, we have proposed the new delivery system for deoxyribozymes in the form of TiO2•PL-Dz nanocomposite. These nanocomposites consist of catalytically active Dz molecules noncovalently immobilized through the polylysine linker to TiO2 nanoparticles, which provide the penetration of Dz into cells. Deoxyribozymes in the prepared TiO2•PL-Dz nanocomposites were shown to retain their ability to cleave RNA targets albeit at a slower rate but with the same site-specificity and similar efficiency as free Dz. Unlike free Dz, the proposed TiO2•PL-Dz nanocomposites penetrate into cells without auxiliary actions.
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Levina S. Asya, Repkova N. Marina, Shikina V. Nadezhda, Ismagilov R. Zinfer, Shatskaya V. Natalia and Zarytova F. Valentina, TiO2~Deoxyribozyme Nanocomposites as Delivery System and Efficient Site-Specific Agents for Cleavage of RNA Targets, Drug Delivery Letters 2014; 4 (2) . https://dx.doi.org/10.2174/2210303104666140222002510
DOI https://dx.doi.org/10.2174/2210303104666140222002510 |
Print ISSN 2210-3031 |
Publisher Name Bentham Science Publisher |
Online ISSN 2210-304X |
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