TiO2~Deoxyribozyme Nanocomposites as Delivery System and Efficient Site-Specific Agents for Cleavage of RNA Targets
Asya S. Levina, Marina N. Repkova, Nadezhda V. Shikina, Zinfer R. Ismagilov, Natalia V. Shatskaya and Valentina F. Zarytova
Affiliation: Institute of Chemical Biology and Fundamental Medicine, Siberian branch of RAS, pr. Lavrent’eva 8, Novosibirsk, 630090, Russia.
Synthetic DNA molecules of 10-23 deoxyribozymes (Dz) are known to be efficient and site-specific agents for
the RNA cleavage in a catalytic manner. To provide the penetration of Dz into cells, we have proposed the new delivery
system for deoxyribozymes in the form of TiO2•PL-Dz nanocomposite. These nanocomposites consist of catalytically active
Dz molecules noncovalently immobilized through the polylysine linker to TiO2 nanoparticles, which provide the
penetration of Dz into cells. Deoxyribozymes in the prepared TiO2•PL-Dz nanocomposites were shown to retain their
ability to cleave RNA targets albeit at a slower rate but with the same site-specificity and similar efficiency as free Dz.
Unlike free Dz, the proposed TiO2•PL-Dz nanocomposites penetrate into cells without auxiliary actions.
Keywords: Delivery, deoxyribozymes, immobilization, nanocomposites, TiO2 nanoparticles.
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