Synergistic Action of the Antifungal β-chitin Binding Protein CBP50 from Bacillus thuringiensis with Bacterial Chitinases
Muhammad Aamer Mehmood, Khadim Hussain, Farooq Latif, Muhammad Rizwan Tabassum, Munazza Gull, Saba Shahid Gill, Anam Saqib and Zahid Iqbal
Affiliation: Department of Bioinformatics and Biotechnology, Faculty of Science & Technology, Government College University Faisalabad, Pakistan.
Purified chitin binding protein the CBP50 from Bacillus thuringiensis and three bacterial chitinases (Chi74,
Chi39 and ChiA) from two different genome sources i.e. Bacillus thuringiensis (Bt) and Serratia proteamaculans, were
used to study the crystalline chitin degradation using different combinations of chitinases with and without chitin binding
protein CBP50. Overall 2-4 folds increase in enzymatic activity of all three chitinases was observed when used in combination
with the CBP50. But the highest increase, 4.64 folds, was observed when combination Chi74+Chi39+CBP50 was
used. Gene loci of all proteins involved in Bt-chitin degradation and protein association network were also analyzed. This
is the first report of synergistic action of Bt-proteins on chitin degradation. Present study will lead us to develop a technology
for environmental friendly conversion of chitin to valuable products.
Keywords: B. thuringiensis, CBP50, chitinases, Non-specific synergistic action.
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