Synergistic Action of the Antifungal β-chitin Binding Protein CBP50 from Bacillus thuringiensis with Bacterial Chitinases

Title:Synergistic Action of the Antifungal β-chitin Binding Protein CBP50 from Bacillus thuringiensis with Bacterial Chitinases

Volume: 11 Issue: 1

Author(s): Muhammad Aamer Mehmood, Khadim Hussain, Farooq Latif, Muhammad Rizwan Tabassum, Munazza Gull, Saba Shahid Gill, Anam Saqib and Zahid Iqbal

Affiliation:

Keywords: B. thuringiensis, CBP50, chitinases, Non-specific synergistic action.

Abstract: Purified chitin binding protein the CBP50 from Bacillus thuringiensis and three bacterial chitinases (Chi74, Chi39 and ChiA) from two different genome sources i.e. Bacillus thuringiensis (Bt) and Serratia proteamaculans, were used to study the crystalline chitin degradation using different combinations of chitinases with and without chitin binding protein CBP50. Overall 2-4 folds increase in enzymatic activity of all three chitinases was observed when used in combination with the CBP50. But the highest increase, 4.64 folds, was observed when combination Chi74+Chi39+CBP50 was used. Gene loci of all proteins involved in Bt-chitin degradation and protein association network were also analyzed. This is the first report of synergistic action of Bt-proteins on chitin degradation. Present study will lead us to develop a technology for environmental friendly conversion of chitin to valuable products.

Cite this article as:

Mehmood Aamer Muhammad, Hussain Khadim, Latif Farooq, Tabassum Rizwan Muhammad, Gull Munazza, Gill Shahid Saba, Saqib Anam and Iqbal Zahid, Synergistic Action of the Antifungal β-chitin Binding Protein CBP50 from Bacillus thuringiensis with Bacterial Chitinases, Current Proteomics 2014; 11 (1) . https://dx.doi.org/10.2174/1570164611666140218231635