A Biological Approach for Color-Stripping of Cotton Fabric Dyed with C.I. Reactive Black 5 Using Fungal Enzymes from Solid State Fermentation
Shahzad A.S. Chatha, Ali I. Mallhi, Abdullah I. Hussain, Muhammad Asgher and Poonam Singh Nigam
Affiliation: School of Biomedical Sciences, Faculty of Life & Health Sciences, University of Ulster, Coleraine, BT52 1SA, UK.
The problem of uneven and faulty dyeing in the finished quality of fabrics is usually tackled through a chemical
stripping process to remove the loose dyes from the surface of dyed cotton fabrics. This research work was undertaken to
investigate the color stripping of cotton fabric dyed with C.I. reactive black 5 in an environmentally-friendly way using
enzymes instead of applying chemicals. Such bio-degradable and economical enzymes were prepared by growing white
rot fungus Ganoderma lucidum IBL-05 under the optimized conditions of solid state fermentation (SSF). The biological
color stripping process using SSF-enzyme extract was optimized for necessary parameters for effective color strippingsuch
as SSF-enzyme concentration, pH, temperature and the duration of the enzyme reaction. The color stripping
efficiency of this enzymatic reaction process was evaluated by measuring the K/S values of dyed fabric before and after
the color stripping. In the present study 70.81% color stripping of dyed fabric was achieved using a cheaper preparation of
crude enzyme extract consisting of three significant enzymes lignin peroxidase (EC 220.127.116.11) (530U/mL), manganese
peroxidase (EC 18.104.22.168) (395U/mL) and laccase (EC 22.214.171.124) (68U/mL) under the optimized reaction conditions of
stripping medium pH 4.5, at 30°Creaction temperature, and 10 days period of stripping. This biological approach is
environmentally friendly as compared to chemical methods used for color stripping in textile industry.
Keywords: Cotton-fabric, reactive-dye, color stripping, ganoderma lucidum IBL-05, solid-state fermentation, lignin
peroxidase, manganese peroxidase, laccase.
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