Determination of Amikacin in Different Pharmaceutical Formulations Using a Resonance Rayleigh Scattering Method with Pontamine Sky Blue
Wenli Han, Fei Qu, Jingchuan Shang, Wei Lu, Junqing Yang and Qing Ma
Affiliation: Department of Pharmacology, Chongqing Key Laboratory of Biochemical and Molecular Pharmacology, Chongqing Medical University, #1 Medical Street, Chongqing 400016, China.
Keywords: Amikacin, resonance Rayleigh scattering, pontamine sky blue, quantitative determination.
Amikacin (AMK) is a semisynthetic aminoglycoside antibiotic derived from kanamycin A that lacks a strong
ultraviolet absorbing chromophore or fluorophore. Greatly enhanced resonance Rayleigh scattering (RRS) signals of
AMK can be observed with a conventional fluorescence spectrophotometer when AMK interacts with pontamine sky blue
(PSB) and forms an ion-association complex in weakly acidic buffer medium. Based on these characteristics, a sensitive
assay for detecting AMK was developed. The maximum RRS peak was observed at 362 nm. The enhancement of the RRS
signal was directly proportional to the AMK concentration in the range of 0–1.7 µg/ml, and its detection limit (3σ) was 3.0
ng/ml. The method showed a wide linear range and high sensitivity, and almost no interference could be observed from
coexistent substances. In addition, this assay was performed in different pharmaceutical formulations of AMK with satisfactory
results. Therefore, the proposed method is promising as an effective means for the determination of AMK.
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