Genotypic and Phenotypic Comparison of Enteroaggregative Escherichia coli Isolates from HIV-Positive and Non-HIV Diarrheal Samples

Title:Genotypic and Phenotypic Comparison of Enteroaggregative Escherichia coli Isolates from HIV-Positive and Non-HIV Diarrheal Samples

Volume: 11 Issue: 8

Author(s): Anis Jafari, Ebrahim Shafaei, Mana Oloomi, Mohammad-Reza Aghasadeghi and Saeid Bouzari

Affiliation:

Keywords: Antibiotic resistance, biofilm, Enteroaggregative Escherichia coli, EAEC, HIV-positive diarrhea, non-HIV diarrhea, virulence-related genes.

Abstract: Enteroaggregative Escherichia coli (EAEC) have been isolated from both HIV-positive and non-HIV diarrheal samples. In this study a collection of 18 isolates from these two groups were compared for biofilm formation and antibiotic resistance and for the presence of 14 virulence-related genes. All the HIV-positive and over 66% of the non- HIV strains were PCR-negative for adhesion-related sequences indicating that as yet unknown adhesins may play a role. However, despite some variations, the prevalence rate of the virulence-related genes was not significantly different in the two groups. HIV-positive isolates were biofilm producer but only a single weak biofilm former was observed among the non-HIV strains. The rate of resistance to most of the antibiotics used was higher among the HIV-positive group than the non-HIV isolates, but was significantly higher for amoxicillin-calvulanic acid (100%) and nalidixic acid (55.5%). Pulse field gel electrophoresis of the isolates produced 17 unique profiles reflecting the exiting heterogeneity of the isolates.

Cite this article as:

Jafari Anis, Shafaei Ebrahim, Oloomi Mana, Aghasadeghi Mohammad-Reza and Bouzari Saeid, Genotypic and Phenotypic Comparison of Enteroaggregative Escherichia coli Isolates from HIV-Positive and Non-HIV Diarrheal Samples, Current HIV Research 2013; 11 (8) . https://dx.doi.org/10.2174/1570162X12666140207161000