Current Drug Discovery Technologies

Vladimir P. Torchilin  
Department of Pharmaceutical Science Center
for Pharmaceutical Biotechnology and Nanomedicine
Northeastern University
Boston, MA 02115
USA

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Different Approaches for Obtaining Antibodies from Human B Cells

Author(s): Marcus R. Duvall and Ryan N. Fiorini

Affiliation: 13 Lowndes Pointe Drive, Charleston, 29403, USA.

Abstract:

Antibodies have emerged as powerful therapeutics effective for treating a number of human conditions and diseases. While early successes utilized small animals to generate therapeutic antibodies, human antibodies are now preferred in order to limit anti-antibody immune responses. Antibodies with human amino acid sequences can be generated in a number of ways, such as humanizing antibodies from other species or expressing human antibodies in transgenic animals. This review focuses on methods for obtaining antibodies directly from human B cells. These methods use both antigen exposed and non-exposed (“naïve”) humans as B cell sources, and apply various technologies to isolate desired antibodies; including cell line generation, single cell isolation, display technologies, and B cell library generation.

Keywords: Antibody engineering, antibody libraries, display technologies, high-throughput screening, human monoclonal antibodies, human B cell.

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Article Details

VOLUME: 11
ISSUE: 1
Page: [41 - 47]
Pages: 7
DOI: 10.2174/1570163811666140121154649