Covalent Immobilization of α-Glucosidase Enzyme onto Amine Functionalized Multi-walled Carbon Nanotubes
A.K.M. Shafiqul Islam,
α-glucosidase (AG) enzyme was immobilized onto amine functionalized multi-walled carbon nanotubes
(MWCNTs-NH2) by treatment with cross linkers, 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) and
N-hydroxysuccinimide (NHS) via amide bonding. The immobilized AG was characterized by Fourier Transform Infrared
Spectroscopy (FTIR) and Scanning Electron Microscope (SEM). The performance of the electrode was evaluated by
Spectrophotometric method and cyclic voltammetric (CV) method using p-nitrophenyl-α-D-glucopyranoside (PNPG) as a
substrate. The immobilized AG enzyme retains about 84.16% activity of its initial response even after 30 days when
stored at 4°C.
Keywords: Cyclic voltammetry, functionalization, α-glucosidase, immobilization.
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