Induced Human Bone Marrow Stromal Cells Differentiate into Neural Cells by bFGF and Cocultured with Olfactory Ensheathing Cells
Wen-Fei Ni, Ai-Min Wu, Qing-Long Li, Zhe-Yu Huang, Hua-Zi Xu and Li-Hui Yin
Affiliation: Institute of Medical Science, The First Affiliated Hospital of Wenzhou Medical University, China.
Bone marrow stromal cells (BMSCs) were considered as one of the strongest candidates for cell transplantations
to treat neurological disorders. Previously, we had showed that BMSCs isolated from rats could be induced to differentiate
into neural cells being cocultured with olfactory ensheathing cells (OECs). In this study, we further demonstrated
the neural differentiation of human BMSCs (hBMSCs) when cocultured with OECs and daily supplement of bFGF (basic
fibroblast growth factor). Transwell culture dishes with a 0.4-mm pore size were used to coculture hBMSCs and OECs. At
different time points (12h, 24h, 3d, 7d, 14d), the induced hBMSCs were morphologically observed and performed immunocytofluorescence
and quantitative RT-PCR (qRT-PCR). The number of neural markers-positive cells significantly increased
after coculture, and gene expression of NSE, β-III-tubulin, MAP2, GFAP also dramatically increased. Our study
suggested that hBMSCs could be induced into neuron-like cells under conditions of coculture with OECs and daily supplement
of bFGF. The differentiated autologous hBMSCs had a great potential for transplantation to treat CNS lesion.
Keywords: Basic fibroblast growth factor, coculture, human bone marrow stromal cells, neural cells, olfactory ensheathing
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