Penetration of Tamoxifen Citrate Loaded Ethosomes and Liposomes Across Human Skin: A Comparative Study with Confocal Laser Scanning Microscopy
Khomendra K. Sarwa, Preeti K. Suresh, Mithun Rudrapal and Vinod K. Verma
Affiliation: University Institute of Pharmacy, Pt. Ravishankar Shukla University, Raipur, Chhattisgarh, India 492010.
Keywords: CLSM, Ethosomes, Liposomes, Tamoxifen citrate.
In the present study, ethosomal and liposomal formulations containing tamoxifen citrate were prepared and
evaluated for their penetration properties in human cadaver skin using Franz diffusion cell and confocal laser scanning
microscope (CLSM). The results clearly revealed that ethosomal vesicles showed a better drug permeation profile than
that of liposomal vesicles. In addition, low fluorescence intensity in CLSM was recorded with liposomes as compared to
ethosomes, indicating lower cumulative amount of drug permeation from liposomal vesicles. Furthermore, CLSM showed
uniform fluorescence intensity across the entire depth of skin in ethosomal treatment, indicating high penetrability of
ethosomal vesicles through human cadaver skin. In contrast, low penetrability of conventional liposomal vesicles was recorded
as penetration was limited to the 7th section (i.e. upper epidermis layer) of skin as evident from visualization of intact
liposomal vesicles in CLSM.
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