Proteomic-based Analysis for Identification of Proteins Involved in 5-fluorouracil Resistance in Hepatocellular Carcinoma

Author(s): Yi Tan, Shukui Qin, Xin Hou, Xiujuan Qian, Jun Xia, Yumei Li, Rui Wang, Changjie Chen, Qingling Yang, Lucio Miele, Qiong Wu, Zhiwei Wang.

Journal Name: Current Pharmaceutical Design

Volume 20 , Issue 1 , 2014

Abstract:

Background: Hepatocellular carcinoma (HCC) has high mortality partly due to acquiring drug resistance during chemotherapy treatment. Therefore, it is necessary to explore the underlying mechanism of drug resistance.

Methods: We used 2-DE and MALDI-TOF-MS analysis to explore the possible molecular insight into 5-FU resistance in HCC. The differentially expressed proteins were validated by Western blot analysis.

Results: We identified 102 unique proteins including p16, maspin, PRDX6, PSMB7, MYL6, PHB, and HSP27 with alteration in SMMC- 7721/5-FU. Furthermore, down-regulation of PRDX6 and PSMB7 enhanced SMMC-7721/5-FU cells to 5-FU sensitivity.

Conclusions: Our study suggests that targeting drug resistant genes such as PRDX6 and PSMB7 could be a novel approach to overcome 5-FU resistance in HCC cells.

Keywords: Hepatocellular carcinoma, chemoresistance, 5-FU, PRDX6, PMSB7.

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Article Details

VOLUME: 20
ISSUE: 1
Year: 2014
Page: [81 - 87]
Pages: 7
DOI: 10.2174/138161282001140113125143
Price: $58

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