Transfection Optimization of Hyperbranched Poly 2-Dimethylaminoethyl Methacrylate (pDMAEMA) based Polymers In Vitro

Author(s): Asha Mathew, Wenxin Wang, Abhay Pandit.

Journal Name: Pharmaceutical Nanotechnology

Volume 2 , Issue 1 , 2014

Become EABM
Become Reviewer

Graphical Abstract:


The present study reports the transfection and cytotoxicity of hyperbranched poly (2-dimethylaminoethylmethacrylate) (pDMAEMA) based polymer at various physiological conditions. Transfection capability and cytotoxicity of the hyperbranched polymer were first evaluated by varying the molecular weight upon PEGylation. Hyperbranched polymer with optimal PEGylation was later protonized and the effect of protonation was evaluated. Finally, the effects of serum on transfection and cellular metabolic activity on various cell types were investigated. Results suggest that hyperbranched polymer with medium PEGylation (Mw 24000Da) and protonation were best suited for transfection which exhibited minimal toxicity. However, the effect of serum was cell-type dependent.

Keywords: Cellular metabolism, PEGylation, poly (2-dimethyl-aminoethylmethacrylate) (pDMAEMA), protonation, transfection.

Rights & PermissionsPrintExport Cite as

Article Details

Year: 2014
Page: [35 - 41]
Pages: 7
DOI: 10.2174/2211738501666140110231305

Article Metrics

PDF: 20