Transfection Optimization of Hyperbranched Poly 2-Dimethylaminoethyl Methacrylate (pDMAEMA) based Polymers In Vitro
Asha Mathew, Wenxin Wang and Abhay Pandit
Affiliation: Network of Excellence for Functional Biomaterials, NFB Building, IDA Business Park, Newcastle Road, Dangan, National University of Ireland, Galway, Ireland.
Keywords: Cellular metabolism, PEGylation, poly (2-dimethyl-aminoethylmethacrylate) (pDMAEMA), protonation, transfection.
The present study reports the transfection and cytotoxicity of hyperbranched poly (2-dimethylaminoethylmethacrylate)
(pDMAEMA) based polymer at various physiological conditions. Transfection capability and
cytotoxicity of the hyperbranched polymer were first evaluated by varying the molecular weight upon PEGylation. Hyperbranched
polymer with optimal PEGylation was later protonized and the effect of protonation was evaluated. Finally,
the effects of serum on transfection and cellular metabolic activity on various cell types were investigated. Results suggest
that hyperbranched polymer with medium PEGylation (Mw 24000Da) and protonation were best suited for transfection
which exhibited minimal toxicity. However, the effect of serum was cell-type dependent.
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