Pharmaceutical Nanotechnology

Ijeoma F. Uchegbu
Department of Pharmaceutics, University of London
London
UK
Email: ijeoma.uchegbu@ucl.ac.uk

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Transfection Optimization of Hyperbranched Poly 2-Dimethylaminoethyl Methacrylate (pDMAEMA) based Polymers In Vitro

Author(s): Asha Mathew, Wenxin Wang and Abhay Pandit

Affiliation: Network of Excellence for Functional Biomaterials, NFB Building, IDA Business Park, Newcastle Road, Dangan, National University of Ireland, Galway, Ireland.

Keywords: Cellular metabolism, PEGylation, poly (2-dimethyl-aminoethylmethacrylate) (pDMAEMA), protonation, transfection.

Graphical Abstract:


Abstract:

The present study reports the transfection and cytotoxicity of hyperbranched poly (2-dimethylaminoethylmethacrylate) (pDMAEMA) based polymer at various physiological conditions. Transfection capability and cytotoxicity of the hyperbranched polymer were first evaluated by varying the molecular weight upon PEGylation. Hyperbranched polymer with optimal PEGylation was later protonized and the effect of protonation was evaluated. Finally, the effects of serum on transfection and cellular metabolic activity on various cell types were investigated. Results suggest that hyperbranched polymer with medium PEGylation (Mw 24000Da) and protonation were best suited for transfection which exhibited minimal toxicity. However, the effect of serum was cell-type dependent.

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Article Details

VOLUME: 2
ISSUE: 1
Page: [35 - 41]
Pages: 7
DOI: 10.2174/2211738501666140110231305