Formulation, Characterization and Ex vivo studies of Terbinafine HCl Liposomes for Cutaneous Delivery
Beeravelli Sudhakar, J.N Ravi Varma and K.V. Ramana Murthy
Background: With the goal of developing effective treatment for dermal fungal infections, delivery of antifungal drugs on skin from liposomal application could be highly beneficial. Topical delivery involves minimizing the flux of the drug through the skin while maximizing its retention in the skin. The aim of the present work was the investigation of the effects of lipids and cholesterol for the development of Terbinafine HCl liposomal formulations as potential carriers for antifungal agents. Phospholipon 90H (Hydrogenated phosphatidylcholine) and Dimyristoylglycero-3- phosphocholine (DMPC) along with cholesterol were used for liposome preparation by ethanol injection method. Drug entrapment, size and morphology were evaluated by UV spectrophotometry, Photon Correlation Spectroscopy (PCS) and transmission electron microscopy (TEM), respectively. Franz diffusion apparatus was used to carry out in vitro, ex vivo drug deposition studies.
Results: Drug entrapment ranged between 39.46 ± 0.91% to 70.39 ± 0.71%. Vesicles showed a narrow size distribution, with size in range of 206.9 to 344.8 nm and a good morphology.
Conclusion: Terbinafine-HCl content was successfully increased with increase in the amount of cholesterol. In vitro and ex vivo comparisons show Terbinafine-HCl liposomes incorporated in gum karaya gel has potentially retained the drug for prolonged period from its release to exhibit the required antifungal activity effectively compared with liposomal dispersion and plain gel
Antifungal, Cholesterol, Liposome, Unilamellar, Cholesterol, Franz Diffusion apparatus, Gum karaya Gel, Terbinafine
Department of Pharmaceutical Technology, A. U. College of pharmaceutical sciences, affiliated Andhra University, Visakhapatnam, Andhra Pradesh, India 530003