Synthesis and Development of MSN-Gd3+-C595 as MR Imaging Contrast Agent for Prostate Cancer Cell Imaging
Bita Mehravi, Mehdi Mirzaei, Mehdi S. Ardestani, Massoud Amanlou, Haleh Soltanghoraee, Negar Ghalandarlaki and Seyed Amir Mohsen Ziaee
Affiliation: Urology and Nephrology Research Center, Shahid Labbafinejad Medical Center, 9th Boustan, Pasdaran Ave, Tehran, Iran.
Purpose: Cell surface antigens as biomarkers suggest high potential for early diagnosis in cancers. The scope of
this study is to synthesize Gd3+ based silica nanoparticles that conjugate on monoclonal antibody C595 by a facile method
in order to detect human prostate cancer cells.
Method: In this study, monoclonal antibody C595, anti-MUC-1, was conjugated on Gd3+-based mesoporous silica nanospheres
(MSN-Gd3+-C595) (71.1 nm) by utilizing N-5-Azido-2-nitrobenzoyloxy succinimide (ANB-NOS) cross linkers.
This contrast agent was characterized using techniques such as nitrogen physisorption, thermogravimetric analysis, scanning
and transmission electron microscopy, inductively coupled plasma atomic emission spectrometry (ICP-AES) and
Dynamic Light Scattering (DLS). The relaxivities were determined using a 3 Tesla MRI scanner. The accurate and easily
available in vitro assay based on MSN-Gd3+-C595 was applied to identify the cell surface antigen expression in the case
of prostate cancer cells and the quantitative data were analyzed.
Results: Results showed that N-5-Azido-2-nitrobenzoyloxysuccinimide cross-linkers were suitable for conjugation of
C595 on the surface of mesoporous silica nanospheres. Protein measurement assay indicated that 8% antibodies were
attached on the surface of mesoporous silica nanospheres and TGA and ICP-AES results demonstrated that 19 wt%
Gd3+-DTTA were loaded into C595-MSN-Gd3+. Furthermore, the obtained data showed a powerful relaxations as well as
selective MUC-1 antigen binding to the cell.
Conclusion: Based on the results of the present study, MSN-Gd3+-C595 nanoprobe may be a potential prostate molecular
imaging and may provide critical guideline in selecting these nanoparticles as an appropriate contrast agent for nanomedicine
Keywords: Prostate cancer cells, gadolinium, Mab C595, mesoporous silica nanospheres, MRI.
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