Current Pharmaceutical Analysis

Anastasios Economou
Department of Chemistry
Laboratory of Analytical Chemistry
University of Athens


Influence of Physical Immobilization of dsDNA on Carbon Based Matrices of Electrochemical Sensors

Author(s): Livia Alexandra Gugoasa, Raluca-Ioana Stefan-van Staden, Anton Alexandru Ciucu and Jacobus Frederick van Staden

Affiliation: Laboratory of Electrochemistry and PATLAB Bucharest, National Institute of Research for Electrochemistry and Condensed Matter, 202 Splaiul Independentei Street, 060021 Bucharest, Romania.


The influence of addition of dsDNA on the electrocatalytic effect of carbon matrices, e.g. synthetic monocrystalline diamond, fullerenes C60 and multi-walled carbon nanotubes was studied. Three neurotransmitters: dopamine, epinephrine and norepinephrine, which are known as biomarkers that play an important role in transporting and modulating nervous impulses in the synapse were chosen as model analytes. Cyclic voltammetry was used to determine the optimum working conditions with a phosphate buffer solution at a pH=4 with KNO3 as support electrolyte. Differential pulse voltammograms (DPVs) were employed to assess the electrolytic effect of dsDNA on carbon matrices under the optimized conditions, and the potential applications of the biosensors for pharmaceutical and biomedical analysis.

Keywords: dsDNA, sensors, carbon based matrices, neurotransmitters.

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Article Details

Page: [20 - 29]
Pages: 10
DOI: 10.2174/157341291001140102104740