Protective Effect of α-ketobutyrate on Survival of Hippocampal Neurons Challenged with Hydrogen Peroxide Chemistry Mimicking Brain Ischemia
Dagmara Jacewicz, Kamila Siedlecka-Kroplewska, Joanna Pranczk, Urszula Popowska, Alicja Kuban-Jankowska, Narcyz Knap, Dariusz Wyrzykowski, Krzysztof Zamojc, Michal Wozniak and Lech Chmurzynski
A protective effect of α-ketobutyrate on hipocampal neuronal cells treated with H2O2 was tested. α-Ketobutyrate concentrations were as follows: 0.5 mM, 1 mM and 5 mM. Tests were performed at the temperature of 37 ºC. Moreover, the protective effect of α-ketobutyrate on the cytotoxicity of 100 and 40 nmol/4x103 cells treated by hydrogen peroxide was observed. Furthermore, the role of α-ketobutyrate as an energy substrate was also proven using the cis-[Cr(C2O4)(pm)(OH2)2]+ complex as a biosensor for the carbon dioxide uptake process. All the experiments were carried out under in-vitro conditions.
Neuronal cells, molecular biosensor of CO2, chromium(III) complex