Current Pharmaceutical Analysis

Anastasios Economou
Department of Chemistry
Laboratory of Analytical Chemistry
University of Athens
Athens
Greece
Email: cpa@benthamscience.org

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Protective Effect of α-ketobutyrate on Survival of Hippocampal Neurons Challenged with Hydrogen Peroxide Chemistry Mimicking Brain Ischemia

Author(s): Dagmara Jacewicz, Kamila Siedlecka-Kroplewska, Joanna Pranczk, Urszula Popowska, Alicja Kuban-Jankowska, Narcyz Knap, Dariusz Wyrzykowski, Krzysztof Zamojc, Michal Wozniak and Lech Chmurzyrski

Affiliation: Faculty of Chemistry, University of Gdansk, Wita Stwosza 63, 80-308 Gdańsk, Poland.

Keywords: Neuronal cells, molecular biosensor of CO2, chromium(III) complex.

Abstract:

A protective effect of α -ketobutyrate on hippocampal neuronal cells treated with H2O2 was tested. α - Ketobutyrate concentrations were as follows: 0.5 mM, 1 mM and 5 mM. Tests were performed at the temperature of 37 ºC. Moreover, the protective effect of α-ketobutyrate on the cytotoxicity of 100 and 40 nmol/4·103 cells treated by hydrogen peroxide was observed. Furthermore, the role of α-ketobutyrate as an energy substrate was also proven using the cis- [Cr(C2O4)(pm)(OH2)2]+ complex as a biosensor for the carbon dioxide uptake process. All the experiments were carried out under in-vitro conditions.

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Article Details

VOLUME: 10
ISSUE: 2
Page: [87 - 91]
Pages: 5
DOI: 10.2174/1573412909666131231231631