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Immunology‚ Endocrine & Metabolic Agents in Medicinal Chemistry
(Formerly Current Medicinal Chemistry - Immunology‚ Endocrine and Metabolic Agents)
ISSN (Print): 1871-5222
ISSN (Online): 1875-5844
Epub Full Text Article
DOI: 10.2174/1871522213999131230123954      Price:  $95









Development of LC-MS/MS Quantification of the Novel Antimicrobial Peptide, SR-0379, and its Pharmacokinetics in Rats

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Author(s): Hideki Tomioka, Hironori Nakagami, Tomokazu Sano, Kazuaki Takafuji, Seiji Takashima, Yasufumi Kaneda and Ryuichi Morishita
Abstract:
During the preclinical study of the original functional peptide, SR-0379, a sensitive liquid chromatographytandem mass spectrometry method was newly developed to study the pharmacokinetics of SR-0379 in rat plasma and subcutaneous tissue samples. Although SR-0379 was unstable in the rat plasma and subcutaneous tissue samples, pretreatment with EDTA and phosphoric acid (4 %) inhibited its degradation. The lower limits of quantification (LLOQ) for SR- 0379 were fully validated as 5 ng/ml in plasma and 5 ng/g in tissue with acceptable linearity, intra- and inter-assay precisions, and accuracy. Measurement of SR-0379 concentration in plasma after intravenous injection via LC-MS/MS yields plasma concentration-time curves (AUC0-
Keywords:
LC-MS/MS, pharmacokinetics, plasma, quantitation, subcutaneous tissue
Affiliation:
Division of Gene Therapy Science, Graduate School of Medicine, Osaka University, 2-2 Yamada-oka, Suita, 565-0871, Japan