Current Catalysis

Shaobin Wang  
Curtin University
Perth, WA 6845 U1987
Australia

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Purification of The Lipase B of Candida antarctica from a Commercial Enzymatic Extract

Author(s): Carlos R. Llerena-Suster, Laura E. Briand and Susana R. Morcelle

Affiliation: Centro de Investigación y Desarrollo en Ciencias Aplicadas-Dr. Jorge J. Ronco. Universidad Nacional de La Plata, CONICET, CCT La Plata. Calle 47 No 257, B1900AJK La Plata, Buenos Aires, Argentina.

Keywords: Protein purification, CALB, UV absorbance, Bradford, size exclusion chromatography, Ion exchange chromatography.

Graphical Abstract:


Abstract:

This paper presents a rational strategy to purify the lipase B of Candida antarctica of the commercial extract Lipozyme® through size exclusion coupled with anionic exchange chromatography using a non conventional, easy to remove buffer system such as ammonia-ammonium. In this context, each step of the purification was followed through the determination of the protein content, esterase activity measurements, SDS-PAGE, agarose electrophoresis, UVspectroscopy and isoelectric focusing. The purification of the commercial extract afforded a sample that retains 47% of the proteins (being CALB the major enzymatic component of the purified sample) with a hydrolytic activity higher than the starting crude extract.

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Article Details

VOLUME: 3
ISSUE: 2
Page: [155 - 160]
Pages: 6
DOI: 10.2174/2211544702666131224234223