Recent Advances in the Post-Column Derivatization for the Determination of Mycotoxins in Food Products and Feed Materials by Liquid Chromatography and Fluorescence Detection
Donatella Nardiello, S. Lo Magro, M. Iammarino, Carmen Palermo, Marilena Muscarella and Diego Centonze
Affiliation: Istituto Zooprofilattico Sperimentale della Puglia e della Basilicata, Via Manfredonia 20, 71100 Foggia, Italy.
The problems and risks associated with mycotoxin contamination of foods and feedstuffs have led to the development
of a variety of confirmatory analytical methods for their determination, based on chromatographic techniques and
In this paper, an overview of the analytical methods developed in the last decade and based on post-column derivatization
coupled with high-performance liquid chromatography and fluorescence detection is reported. A particular focus on the
determination of aflatoxins (B1, B2, G1 and G2), fumonisins (B1 and B2) and trichotecenes deoxynivalenol (DON) and nivalenol
(NIV) in cereals products for human and animal consumption is given. Important aspects as the optimization of
the experimental conditions and validation of the analytical methods are also described.
Keywords: Aflatoxins, fumonisins, fluorescence detection, DON, liquid chromatography, method validation, NIV, postcolumn
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