Microarray Technologies for Intracellular Kinome Analysis
T. Yamamoto, T. Mori and Y. Katayama
Affiliation: Department of Applied Chemistry, Kyushu University, 744 Moto-oka, Nishi-ku, Fukuoka 819- 0395, Japan.
Microarray-based kinomics, which measure the enzymatic activity or the presence of intracellular protein
kinases, are now regarded as alternative tools to conventional mass spectrometry-based kinomics for examining intracellular
kinomics. Here, we reviewed the principal advantages, recent progress, and remaining problems of representative microarray-
based kinomics, including substrate peptide and protein microarrays, anti-protein kinase antibody microarrays,
and reverse protein microarrays. Microarray-based kinomics are not as good at quantitative evaluation of kinomics as the
conventional mass spectrometry-based kinomics. However, their simplicity and high throughput make the microarraybased
kinomics unique tools, being especially suited for a practical analysis; monitoring drug effects on cellular kinomics
as a tool for drug development, and for the diagnosis and prognosis of diseases based on kinomics.
Keywords: Antibody, companion diagnosis, diagnosis, drug development, drug screening, intracellular signaling, microarray,
molecular targeted drug, order-made medicine, peptide, personalized medicine, prognosis, protein, protein kinase.
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