Microarray Technologies for Intracellular Kinome Analysis

Author(s): T. Yamamoto, T. Mori, Y. Katayama.

Journal Name:Current Medicinal Chemistry

Volume 21 , Issue 22 , 2014

Abstract:

Microarray-based kinomics, which measure the enzymatic activity or the presence of intracellular protein kinases, are now regarded as alternative tools to conventional mass spectrometry-based kinomics for examining intracellular kinomics. Here, we reviewed the principal advantages, recent progress, and remaining problems of representative microarray- based kinomics, including substrate peptide and protein microarrays, anti-protein kinase antibody microarrays, and reverse protein microarrays. Microarray-based kinomics are not as good at quantitative evaluation of kinomics as the conventional mass spectrometry-based kinomics. However, their simplicity and high throughput make the microarraybased kinomics unique tools, being especially suited for a practical analysis; monitoring drug effects on cellular kinomics as a tool for drug development, and for the diagnosis and prognosis of diseases based on kinomics.

Keywords: Antibody, companion diagnosis, diagnosis, drug development, drug screening, intracellular signaling, microarray, molecular targeted drug, order-made medicine, peptide, personalized medicine, prognosis, protein, protein kinase.

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Article Details

VOLUME: 21
ISSUE: 22
Year: 2014
Page: [2542 - 2552]
Pages: 11
DOI: 10.2174/0929867321666131212154153
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