Abstract
The purpose of the study was to evaluate the expression of CD45, CD34, Sca-1 and CD117 in mouse bone marrow, Lin-ve and Lin+ve population. Bone marrow cells were isolated from C57/BL6J mouse and mononuclear population was separated from rest of the cell population. With the help of Magnetic associated cell sorter (MACS), Linve and Lin+ve cells were separated from the bone marrow. The expression of CD45, CD34, Sca1 and CD117 was evaluated in bone marrow, Lin-ve and Lin+ve population by flow cytometry. We found a significant increase in the expression of CD34 and CD117 in Lin-ve as compared to the bone marrow and Lin+ve population. These findings suggest that Lin-ve population has higher expression of stem cell progenitor markers and could be useful for tissue repair and regeneration.
Keywords: Bone marrow, CD34, CD45, CD117, EPCs, Sca1, Stem cell.
Current Neurovascular Research
Title:Characterization of Lin-ve CD34 and CD117 Cell Population Reveals an Increased Expression in Bone Marrow Derived Stem Cells
Volume: 11 Issue: 1
Author(s): Neeru Jindal, Gillipsie Minhas, Sudesh Prabhakar and Akshay Anand
Affiliation:
Keywords: Bone marrow, CD34, CD45, CD117, EPCs, Sca1, Stem cell.
Abstract: The purpose of the study was to evaluate the expression of CD45, CD34, Sca-1 and CD117 in mouse bone marrow, Lin-ve and Lin+ve population. Bone marrow cells were isolated from C57/BL6J mouse and mononuclear population was separated from rest of the cell population. With the help of Magnetic associated cell sorter (MACS), Linve and Lin+ve cells were separated from the bone marrow. The expression of CD45, CD34, Sca1 and CD117 was evaluated in bone marrow, Lin-ve and Lin+ve population by flow cytometry. We found a significant increase in the expression of CD34 and CD117 in Lin-ve as compared to the bone marrow and Lin+ve population. These findings suggest that Lin-ve population has higher expression of stem cell progenitor markers and could be useful for tissue repair and regeneration.
Export Options
About this article
Cite this article as:
Jindal Neeru, Minhas Gillipsie, Prabhakar Sudesh and Anand Akshay, Characterization of Lin-ve CD34 and CD117 Cell Population Reveals an Increased Expression in Bone Marrow Derived Stem Cells, Current Neurovascular Research 2014; 11 (1) . https://dx.doi.org/10.2174/1567202610666131209110035
DOI https://dx.doi.org/10.2174/1567202610666131209110035 |
Print ISSN 1567-2026 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5739 |
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
Related Articles
-
Effects of Coenzyme Q and Creatine Supplementation on Brain Energy Metabolism in Rats Exposed to Chronic Cerebral Hypoperfusion
Current Alzheimer Research Cardiovascular Molecular Imaging: New Methodological Strategies
Current Pharmaceutical Design Non-Transferrin Bound Iron - Determination in Biological Material and Clinical Implications
Current Pharmaceutical Analysis Amniotic Fluid Stem Cells: a Promising Therapeutic Resource for Cell-Based Regenerative Therapy
Current Pharmaceutical Design Renal Ischemia: How Commonly Does it Cause Renal Failure?
Current Hypertension Reviews Apoptosis in Anthracycline Cardiomyopathy
Current Pediatric Reviews A Series of Novel Neuroprotective Blood Brain Barrier Penetrating Flavonoid Drugs to Treat Acute Ischemic Stroke
Current Pharmaceutical Design Therapeutic Effects of Progesterone in Animal Models of Neurological Disorders
CNS & Neurological Disorders - Drug Targets S-nitrosation/Denitrosation in Cardiovascular Pathologies: Facts and Concepts for the Rational Design of S-nitrosothiols
Current Pharmaceutical Design Recent Patents on Topical Application of Honey in Wound and Burn Management
Recent Patents on Inflammation & Allergy Drug Discovery Fullerenes: From Carbon to Nanomedicine
Mini-Reviews in Medicinal Chemistry Anti-inflammatory Property of AMP-activated Protein Kinase
Anti-Inflammatory & Anti-Allergy Agents in Medicinal Chemistry Mechanism and Inhibition of Matrix Metalloproteinases
Current Medicinal Chemistry The Role of Oxidative Stress and Anti-Oxidant Treatment in Platinum- Induced Peripheral Neurotoxicity
Current Cancer Drug Targets Use of Diffusion- and Perfusion-Weighted Magnetic Resonance Imaging in Drug Development for Ischemic Stroke
Current Drug Targets - CNS & Neurological Disorders Oxidative Stress in Traumatic Brain Injury
Current Medicinal Chemistry Neuroprotection by Natural Polyphenols: Molecular Mechanisms
Central Nervous System Agents in Medicinal Chemistry Reactive Oxygen and Nitrogen Species in the Renal Ischemia/Reperfusion Injury
Current Pharmaceutical Design Modeling of 2-Pyridin-3-yl-Benzo[d][1,3]Oxazin-4-one Derivatives by Several Conformational Searching Tools and Molecular Docking
Current Pharmaceutical Design Human Carbonyl Reductases
Current Drug Metabolism