Metabolic Evidence of Diminished Lipid Oxidation in Women With Polycystic Ovary Syndrome

Author(s): Leah D. Whigham , Daniel E. Butz , Hesam Dashti , Marco Tonelli , LuAnn K. Johnson , Mark E. Cook , Warren P. Porter , Hamid R. Eghbalnia , John L. Markley , Steven R. Lindheim , Dale A. Schoeller , David H. Abbott , Fariba M. Assadi-Porter .

Journal Name: Current Metabolomics

Volume 1 , Issue 4 , 2013

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Abstract:

Polycystic ovary syndrome (PCOS), a common female endocrinopathy, is a complex metabolic syndrome of enhanced weight gain. The goal of this pilot study was to evaluate metabolic differences between normal (n=10) and PCOS (n=10) women via breath carbon isotope ratio, urinary nitrogen and nuclear magnetic resonance (NMR)- determined serum metabolites. Breath carbon stable isotopes measured by cavity ring down spectroscopy (CRDS) indicated diminished (p<0.030) lipid use as a metabolic substrate during overnight fasting in PCOS compared to normal women. Accompanying urinary analyses showed a trending correlation (p<0.057) between overnight total nitrogen and circulating testosterone in PCOS women, alone. Serum analyzed by NMR spectroscopy following overnight, fast and at 2 h following an oral glucose tolerance test showed that a transient elevation in blood glucose levels decreased circulating levels of lipid, glucose and amino acid metabolic intermediates (acetone, 2-oxocaporate, 2-aminobutyrate, pyruvate, formate, and sarcosine) in PCOS women, whereas the 2 h glucose challenge led to increases in the same intermediates in normal women. These pilot data suggest that PCOS-related inflexibility in fasting-related switching between lipid and carbohydrate/protein utilization for carbon metabolism may contribute to enhanced weight gain.

Keywords: Cavity ring down, glucose elevation, lipid metabolism, NMR-metabolomics, polycystic ovary syndrome (PCOS).

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Article Details

VOLUME: 1
ISSUE: 4
Year: 2013
Page: [269 - 278]
Pages: 10
DOI: 10.2174/2213235X01666131203230512

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