Metabolic Evidence of Diminished Lipid Oxidation in Women With Polycystic Ovary Syndrome
Leah D. Whigham, Daniel E. Butz, Hesam Dashti, Marco Tonelli, LuAnn K. Johnson, Mark E. Cook, Warren P. Porter, Hamid R. Eghbalnia, John L. Markley, Steven R. Lindheim, Dale A. Schoeller, David H. Abbott and Fariba M. Assadi-Porter
Affiliation: Department of Nutritional and Human Health Sciences, Texas Tech University, 1301 Akron Ave, Lubbock, TX, 79409, USA.
Polycystic ovary syndrome (PCOS), a common female endocrinopathy, is a complex metabolic syndrome of
enhanced weight gain. The goal of this pilot study was to evaluate metabolic differences between normal (n=10) and
PCOS (n=10) women via breath carbon isotope ratio, urinary nitrogen and nuclear magnetic resonance (NMR)-
determined serum metabolites. Breath carbon stable isotopes measured by cavity ring down spectroscopy (CRDS) indicated
diminished (p<0.030) lipid use as a metabolic substrate during overnight fasting in PCOS compared to normal
women. Accompanying urinary analyses showed a trending correlation (p<0.057) between overnight total nitrogen and
circulating testosterone in PCOS women, alone. Serum analyzed by NMR spectroscopy following overnight, fast and at 2
h following an oral glucose tolerance test showed that a transient elevation in blood glucose levels decreased circulating
levels of lipid, glucose and amino acid metabolic intermediates (acetone, 2-oxocaporate, 2-aminobutyrate, pyruvate, formate,
and sarcosine) in PCOS women, whereas the 2 h glucose challenge led to increases in the same intermediates in
normal women. These pilot data suggest that PCOS-related inflexibility in fasting-related switching between lipid and
carbohydrate/protein utilization for carbon metabolism may contribute to enhanced weight gain.
Keywords: Cavity ring down, glucose elevation, lipid metabolism, NMR-metabolomics, polycystic ovary syndrome (PCOS).
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