Highly Sensitive and Simple Validated Ultra-performance Liquid Chromatography/ Tandem Mass Spectrometry Method for the Determination of Cinacalcet in Human Plasma
Tanveer A. Wani,
Nasr Y. Khalil,
Ahmed H. Bakheit.
With the objective of decreasing analysis time and retaining good efficiency (UPLC-MS/MS) is an outstanding
analytical approach for speedy biomedical analysis. The aim of this study was to develop validate a simple, rapid, sensitive
and specific UPLC-MS/MS method for quantification of cinacalcet, a calcimimetic agent, which acts on a calcium
sensing receptor of the parathyroid gland in human plasma. After following a liquid–liquid extraction procedure with diethyl
ether–dichloromethane (70:30, v/v), cinacalcet (CIN) and internal standard (IS) abiraterone with C18 Acquity UPLC
BEHTM column. m/z 358.07 > 155.0 and m/z 350.1 > 156.0 where the ion transitions recorded in the positive ion multiple
reaction monitoring mode for cinacalcet for IS. The mobile phase consisted of acetonitrile: 10 mM ammonium acetate:
formic acid (90:10:0.1% v/v/v) with a flow rate of 0.4 mL/min. The assay exhibited a linear dynamic range of 0.2–100
ng/mL for cinacalcet in human plasma with good correlation coefficient (IQ 0.995) and with an LOQ of 1 ng/mL. The intra-
and inter-assay precisions were satisfactory; the relative standard deviations did not exceed 6.98 %. This method was
simple, rapid and highly sensitive, hence could be used for analysis of cinacalcet in human plasma.
Keywords: Cinacalcet, UPLC, LC-MS/MS, pharmacokinetic study, human plasma.
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