Bacterial cellulose (BC) has become established as a remarkably versatile biomaterial and can be used in a
wide variety of applied scientific applications, especially for medical devices. In this work, the bacterial cellulose fermentation
process is modified by the addition of hyaluronic acid and gelatin (1% w/w) to the culture medium before the bacteria
is inoculated. Hyaluronic acid and gelatin influence in bacterial cellulose was analyzed using Transmission Infrared
Spectroscopy (FTIR) and Scanning Electron Microscopy (SEM). Adhesion and viability studies with human dental pulp
stem cells using natural bacterial cellulose/hyaluronic acid as scaffolds for regenerative medicine are presented for the
first time in this work. MTT viability assays show higher cell adhesion in bacterial cellulose/gelatin and bacterial cellulose/
hyaluronic acid scaffolds over time with differences due to fiber agglomeration in bacterial cellulose/gelatin. Confocal
microscopy images showed that the cell were adhered and well distributed within the fibers in both types of scaffolds.
Keywords: Bacterial cellulose, cell viability study, nanoskin®, natural nanocomposites, regenerative medicine, stem cells.
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