Current Stem Cell Research & Therapy

Anthony Atala  
Wake Forest University School of Medicine,
Medical Center Boulevard
Winston Salem, NC 27157


Novel Chemically Modified Bacterial Cellulose Nanocomposite as Potential Biomaterial for Stem Cell Therapy Applications

Author(s): Gerson Arisoly Xavier Acasigua, Gabriel Molina de Olyveira, Ligia Maria Manzine Costa, Daikelly Iglesias Braghirolli, Anna Christina Medeiros Fossati, Antonio Carlos Guastaldi, Patricia Pranke, Gildásio de Cerqueira Daltro and Pierre Basmaji

Affiliation: Department of Physical Chemistry- UNESP/Araraquara-SP, 14800-900, Brazil.

Keywords: Bacterial cellulose, cell viability study, nanoskin®, natural nanocomposites, regenerative medicine, stem cells.


Bacterial cellulose (BC) has become established as a remarkably versatile biomaterial and can be used in a wide variety of applied scientific applications, especially for medical devices. In this work, the bacterial cellulose fermentation process is modified by the addition of hyaluronic acid and gelatin (1% w/w) to the culture medium before the bacteria is inoculated. Hyaluronic acid and gelatin influence in bacterial cellulose was analyzed using Transmission Infrared Spectroscopy (FTIR) and Scanning Electron Microscopy (SEM). Adhesion and viability studies with human dental pulp stem cells using natural bacterial cellulose/hyaluronic acid as scaffolds for regenerative medicine are presented for the first time in this work. MTT viability assays show higher cell adhesion in bacterial cellulose/gelatin and bacterial cellulose/ hyaluronic acid scaffolds over time with differences due to fiber agglomeration in bacterial cellulose/gelatin. Confocal microscopy images showed that the cell were adhered and well distributed within the fibers in both types of scaffolds.

Order Reprints Order Eprints Rights & PermissionsPrintExport

Article Details

Page: [117 - 123]
Pages: 7
DOI: 10.2174/1574888X08666131124135654