Mechanisms and Signals for the Nuclear Import of Proteins

Pp. 77-100 (24)

Natália Freitas and Celso Cunha

Abstract

In eukaryotes, the nuclear membrane provides a physical barrier to the passive diffusion of macromolecules from and into the cytoplasm. Nucleocytoplasmic traffic occurs through highly specialized structures known as nuclear pores, and involves the participation of a special class of transport proteins. Active transport across the nuclear pores is an energy-dependent process that relies on the activity of Ran- GTPases both in the nuclear and cytoplasmic compartments.

Nuclear import of proteins is an essential step in regulating gene expression and the replication cycle of several viruses. In this review, the key mechanisms, pathways, and models underlying the transport of proteins across nuclear pores are analysed.

Keywords:

Nuclear pore complex, nucleoporin, nuclear localization signal, protein import, importin, karyopherin.

Affiliation:

Unidade de Microbiologia Médica, Centro de Malária e outras Doenças Tropicais, Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa, Rua da Junqueira, 100 1349-008 Lisboa, Portugal.