Improvements in genomic sequencing technology have accelerated the accumulation of gene information,
leading to the emergence of proteomics as a powerful tool to study the functional genome. Specifically, the emergence of
two technologies, two-dimensional gel electrophoresis (2-DE) and high-throughput protein identification using mass
spectrometry (MS), was a milestone in the development of proteomics. Due to bacteria having a simple genomic system
and ease of sample preparation, these organisms were rapidly subjected to proteomic analyses. Bacterial proteomics is
considered complementary to genomic analyses, including full-genome sequencing and transcriptomics. Proteomics has
revealed novel and valuable information on gene products (i.e., proteins), including their translation level, posttranslational
modifications, turnover, and localization. Recently, the proteomic approach was applied to the study of
extremophiles. In this review, we briefly summarize recent proteomic technologies applicable to bacteria and archea
extremophiles and review the literature describing proteomic research in these organisms. Finally, we discuss future
perspectives on the use of proteomics to study extremophiles.
Archaeal proteomics, bacterial proteomics, extremophiles, gel-based proteomics, hydrothermophilic, liquid
chromatography-based proteomics, mass spectrometry, membrane vesicle, metaproteomics.
Division of Life Science, Korea Basic Science Institute, Daejeon 305-806, Republic of Korea.